Our Fluorescence Microscopes

Live Cell Incubator Microscope

Live Cell Imaging with Your Incubator Microscope

With Etaluma’s high resolution, versatile, and compact inverted fluorescence microscopes that actually fit inside your incubator, you get stable temperatures and CO2 levels throughout your time-lapse live cell imaging assays.
  • Quality comparable to traditional, high-cost wide field fluorescence microscopes
  • Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
  • 3-Color fluorescence, phase contrast, brightfield
  • Images, time-lapse & live video
  • 3d live cell imaging
  • 1.25-100x objectives
  • LS850 pictured above with phase contrast: Auto XY stage & autofocus

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LS820 Microscope

Blue, Green, and Red Fluorescence Microscopy with Autofocus and Z-Stacking for Time Lapse Automation

The powerful, new LS820 Microscope is the latest generation of our fully automated three-channel motorized Z model and offers the latest advances in optics, camera, and user flexibility. The LS820 provides the image quality, Z-range, illumination, and software flexibility over the manual LS620 model. Motorized focus allows autofocus and z-stacks over 14 mm with 100 nm steps. Easy-to-configure software generating user readable and editable scripts, facilitate your automated microscopy experiments. The LS820 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging flasks, dishes or slides with 3 fluorophores in a multi-day time-lapse, the LS820 offers a compact solution for walk-away automated microscopy!

LS620
LS850 Microscope
Blue, Green, & Red Fluorescence Microscopy and Walk-Away Automation

Powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and offers the latest advances in optics, cameras, throughput, and user flexibility. . The LS850 improves the image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your automated microscopy experiments.

The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!

LS720
Features and Benefits
  • Automated XY stage with autofocus in Z provides images (photos), time-lapse series, and videos recorded directly to your computer
  • Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
  • Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
  • Robust software allows set-up and control across many locations, including microplates and custom arrays
  • Versatile and compact design enables use inside cell culture incubators and hoods
  • Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
  • Flip-up deck allows easy objective access
  • Used manually, but also robot compatible (RS485, RS232, 5V digital interfaces)
  • Objective compatibility with standard lenses permits use of your own objectives

Both utilize a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. 

Excitation and Emission Profiles of LS850 and LS820 filters
Semrock 620 chart
Comparison of Lumascope Models
FeaturesLS820LS850
OpticsBlue, green & red fluorescence;
bright field
Blue, green & red fluorescence;
bright field
Phase ContrastPhase contrast optional (Auraphase)Phase contrast optional (Auraphase)
Objective Options1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersBlue: Ex 370-410, Em 429-462 nm;
Green: Ex 473-491, Em 502-561 nm;
Red: Ex 580-598, Em 612-680 nm
Blue: Ex 370-410, Em 429-462 nm;
Green: Ex 473-491, Em 502-561 nm;
Red: Ex 580-598, Em 612-680 nm
CameraHigh QE Monochrome CMOS BSI Sensor; 4.4 Megapixel, 12-BitHigh QE Monochrome CMOS BSI Sensor;
4.4 Megapixel, 12-Bit
Image FormatsJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNG
Image Size100x100 to 2100x2100 pixels100x100 to 2100x2100 pixels
Field of ViewUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20x
Video Rates45 fps (exposure limited)45 fps (exposure limited)
StageManual XYAutomated XY, 110 mm X 74mm, 1-3 micron positional reproducibility
Motorized Z14mm travel, 100nm step, image-based autofocus, Z-stacks14mm travel, 100nm step, image-based autofocus, Z-stacks
Well-to-well time (seconds)NAImage 96 wells: under 2 minutes with
1 channel, no auto-focus
Control SoftwareLumaviewProLumaviewPro
Computer RequirementsWindows 10, 11; Core i7, SSDWindows 10, 11; Core i7, 1 TB SSD, 8 GM RAM
Integration Python Source under the MIT
Open Source License
Python Source under the MIT
Open Source License
Power RequirementsUSB3 for LS; 100-240 V, 50-60 Hz for autostageUSB3 for LS; 100-240 V, 50-60 Hz for autostage
Dimensions (WxDxH)24cm W x 22.6cm D x 27.8cm H
(9.4in W x 8.9in D x 10.9in H)
39.8cm W x 29.7cm D x 29.7cm H
(15.6in W x 11.6in D x 11.6in H)
Weight5 kg (10 lb)9.5 kg (21 lb)
Operating Conditions0°C - 42°C, 5% - 95% RH
non-condensing
0°C - 42°C, 5% - 95% RH
non-condensing

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