Lumascopes
Applications
Applications
Lumascopes can be used in practically any application where a standard inverted (and many upright) microscopes are used. Most of the applications below involve cells but Lumascopes can also image beads and other materials.
Time-lapse video of HT1080 cells expressing cell cycle proteins on an LS600; provided by Alex Zambon, Keck Graduate Institute (see Video Gallery for more info)
See Etaluma – Cardiac Myocytes Undergoing Division
Cell growth and confluence
See Time Lapse Video of MSC in 2D Cell Culture
Cell migration and wound healing
See Cell Migration & Wound Healing Application Note
See Migration of MSC in 2D Cell Culture
Cell cycle protein expression
See Human HT1080 Fibrosarcoma Cells with LS600
Use of micro-environmental systems
See Bioptechs products on Etaluma LS500
Calcium assays
GCAMP5 activity in a sensory neuron
Determining transfection efficiency
In Vitro Exercise Model
Cultured skeletal muscle myotubes are electrically stimulated under hypoxic conditions and with temperature manipulations. Cell signal transduction dynamics are measured using proteomic techniques to help understand how exercise stressors are translated into fitness-promoting adaptions such as increased mitochondria. Probe in photo measures PO2 in the cell medium rather than in the atmosphere. LS620 allows visualization of contracting cells and assessment of their health.
Thank you to Dr David Clarke and his lab, Laboratory for Quantitive Exercise Biology, Simon Fraser University, British Columbia, Canada
Behavior of stem cells
See Etaluma-Human Neural Stem Cells in Culture 1
See Etaluma-Human Neural Stem Cells in Culture 2
Also see reattachment of neuronal stem cells passaged with Accutase (scroll down to see video)
Cell death assays
Apoptosis induction
Spheroid development and behavior
See 3D Spheroid Formation of MSC
See Spheroid-Migration of MSC in a PEG-Fibrinogen Hydrogel
Cultivation of yeast
See Cultivation of S. cerevisiae in Core-Shell Microcapsules
Intravital studies
See Series: Neutrophil migration intravital mouse imaging
Study of lower eukaryotes
Photomicroscopy in locations without AC power
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