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Our Microscopes

LS820 Microscope

The LS820 offers autofocus and Z-stacks.

Blue, Green,
& Red Fluorescence with Autofocus and Z-Stacking for Time Lapse Automation

The powerful, new LS820 Microscope is the latest generation of our fully automated three-channel motorized Z model and offers the latest advances in optics, camera, and user flexibility. The LS820 provides the image quality, Z-range, illumination, and software flexibility over the manual LS620 model. Motorized focus allows autofocus and z-stacks over 14 mm with 100 nm steps. Easy-to-configure software generating user readable and editable scripts, facilitate your automated microscopy experiments. The LS820 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging flasks, dishes or slides with 3 fluorophores in a multi-day time-lapse, the LS820 offers a compact solution for walk-away automated microscopy!

Lumascope LS850

Features and Benefits

Lumascope LS850 In Incubator
  • Images, Z-stacks, time-lapse series, and videos recorded directly to your computer

  • Bright field and phase contrast transmitted illumination
  • LED excitation and high performance optics provide near diffraction-limited resolution
  • Multi-OS software runs on Windows, Mac and Linux
  • Open deck allows microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers
  • Long range motorized Z for autofocus and Z-stacks down to 100 nm
  • Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and on the bench
  • Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry
  • Open source Python control code available
  • Objective compatibility with standard lenses

Like all of Etaluma’s microscopes, the LS820 utilizes a Semrock Brightline® Pinkel filter set optimized for our 405 nm, 488 nm, and 590 nm LED light sources. The 5-filter set is comprised of 3 excitation filters, 1 triple-band dichroic, and 1 triple-band emission filter. The LS820 use a 12 bit monochrome CMOS sensor.

Excitation and Emission Profiles of LS820 filters

Semrock 620 chart
3-color low mag 280
LS820 Microscope Specifications
LED Excitation:405 nm, 488 nm, and 589 nm
Fluorescence Filters:Blue: Excitation 370-410 nm, Emission 429-462 nm
Green: Excitation 473-491 nm, Emission 502-561 nm
Red: Excitation 580-598 nm, Emission 612-680 nm
Transmitted Modes:Bright Field, Phase Contrast
Objectives:1.25x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil)
Camera:High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit
Image Formats:JPG, BMP, TIF, or PNG
Image Size:Adjustable up to 2100 x 2100 pixels
Video Rates:25 FPS (exposure limited)
Motorized Z:14mm travel, 100 nm step, image-based autofocus, Z-stacks
Control Software:LumaviewPro, multi operating system application
Computer Requirements:Windows 10, 11; Core i7, 512TB SSD, 8GB
RAM MacOS with M1; 512TB SSD, 8GB RAM
Linux Debian Distributions:512TB SSD, 8GB RAM
Power Requirements:80-264 V, 50-60 Hz 10W typical, 40W Max
Integration:Python source under the MIT Open Source License
Dimensions:22 cm x 14 cm x 11 cm
Weight:5 Kg/11 lbs
Operating Conditions:0°C - 42°C, 5% - 95% RH non-condensing
Lumascope 820 Objective

Uses commercial microscope objectives

etaluma-lumascope-820-05-800ph-02a

Multi-mode transmitted illumination technology

etaluma-lumascope-820-05-LS820-03a

Accommodates almost any sample

Lumascope 820 LV Pro GUI preview

LumaviewPro application runs on
Windows, Mac, and Linux

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