Our Microscopes
LS850 Microscope
The LS850 offers a whole new world of automated microscopy.
Blue, Green, and Red Fluorescence and Walk-Away Automation
Powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and offers the latest advances in optics, cameras, throughput, and user flexibility. . The LS850 improves the image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your automated microscopy experiments.
The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!
Features and Benefits
- Images, tiled images, Z-stacks, time-lapse series, and videos recorded directly to your computer
- Bright field and phase contrast transmitted illumination
- LED excitation and high performance optics provide near diffraction-limited (theoretical maximum) resolution
- Multi-OS software allows set-up and control across any locations, including microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers and custom arrays
- High speed stage with single digit micron precision
- Long range motorized Z for autofocus and Z-stacks down to 100 nm
- Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and gloveboxes
- Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry GFP
- Open source Python control code available
- Objective compatibility with standard lenses and available with optional automated 4-objective turret
Like all of microscopes, the LS850 utilizes a Chroma Pinkel filter set optimized for 405 nm, 488 nm, and 590 nm LED light sources. The 7-filter set is comprised of excitation filters, dichroics, and an emission filter. The LS850 use a 12 bit monochrome CMOS sensor.
The new LS800 series microscopes are equipped with a low profile, multimodal illuminator that was developed in collaboration with Cairn Research Ltd (based on Aura/Aura Pro™ phase contrast illuminator, based on work by Dr Kevin Webb, Optics & Photonics Research Group, University of Nottingham: Webb, Kevin F. “Condenser‐free contrast methods for transmitted‐light microscopy.” Journal of microscopy 257.1 (2015): 8-22.
Excitation and Emission Profiles of LS850 Filters
| LS850 Microscope Specifications | |
|---|---|
| LED Excitation: | 405 nm, 488 nm, and 589 nm |
| Optics: | DAPI, FITC, mCherry; Optional: FITC, TRITC, Cy5; Phase contrast, brightfield |
| Fluorescence Filters: | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
| Phase Contrast: | Phase contrast (Auraphase) |
| Objective Options: | 1.25x, 2.0x, 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) |
| Objective Compatibilities: | RMS-threaded, infinity corrected, 45 mm parfocal distance |
| Camera: | High Sensitivity Monochrome CMOS BSI Sensor; 5 MP 12-Bit |
| Image Formats: | TIFF, OME-TIFF, ImageJ Hyperstack |
| Image Size: | Adjustable up to 2100 x 2100 pixels |
| Field of View: | Up to 0.78 x 0.78 mm at 20x |
| Stage: | 120 mm x 80mm travel, 1-3 micron positional repeatability |
| Time to Image 96 Well Plate: | 65 seconds: single channel; no autofocus |
| Well-to-well time (seconds): | Image 96 wells: 65 seconds with 1 channel, no auto-focus |
| Motorized Z: | 14mm travel, 100 nm step, image-based autofocus, Z-stacks |
| Control Software: | LumaviewPro, multi operating system application |
| Computer Requirements: | Windows 11; Core i7, 512TB SSD, 8GB RAM MacOS with M1; 512TB SSD, 8GB RAM |
| Power Requirements: | USB3 for LS; 100-240 V, 50-60 Hz for autostage |
| Integration: | Python Source under the MIT Open Source License |
| Dimensions (WxDxH): | 15.6in W x 11.6in D x 11.6in H (39.8cm W x 29.7cm D x 29.7cm H) |
| Weight: | 21 lb (9.5kg) |
| Operating Conditions: | 0°C - 42°C, 5% - 95% RH non-condensing |
Uses commercial microscope objectives
Multi-mode transmitted illumination technology
Optional motorized 4-position turret
LumaviewPro application runs on Windows, Mac and Linux
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