Can you see what’s really happening with your cells? With Etaluma’s high resolution, versatile, and compact inverted microscopes that actually fit inside your incubator, you get stable temperatures and CO2 levels throughout your assays.
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Or call 760-298-2355.
- Quality comparable to traditional, high-cost microscopes
- Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
- 3-Color fluorescence, phase contrast, brightfield
- Images, time-lapse & live video
- 1.25-100x objectives
- LS720 pictured above with phase contrast: Auto XY stage & autofocus
Blue, Green, & Red Fluorescence and Walk-Away Automation
The powerful, new Lumascope 720 (LS720) adds walk-away automation to the many features and high performance of the flagship 3-color LS620. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your microscopy experiments and high content screens. Place the LS720 in your incubator and you have a live cell imaging system at a fraction of the cost of conventional HCS systems. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a 48 hour time-lapse, the 720 offers a whole new world of automated microscopy!
Features and Benefits
- Automated XY stage with autofocus in Z provides images (photos), time-lapse series, and videos recorded directly to your computer
- Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
- Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
- Robust software allows set-up and control across many locations, including microplates and custom arrays
- Versatile and compact design enables use inside cell culture incubators and hoods
- Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
- Flip-up deck allows easy objective access
- Used manually, but also robot compatible (RS485, RS232, 5V digital interfaces)
- Objective compatibility with standard lenses permits use of your own objectives
Like the LS620, it utilizes a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The 720 also contains the same CMOS sensor as the LS620 that is larger than the sensor used in Lumascopes 500 and 400.
Excitation and Emission Profiles of Lumascope 720 filters
| LS720 Microscope Specifications | |
|---|---|
| Optics | Blue, green & red fluorescence; brightfield |
| Phase Contrast | Phase contrast included |
| Objective Options | 2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
| Objective Compatibilities | RMS-threaded, infinity corrected, 45 mm parfocal distance |
| Fluorescence Filters | Blue: Excitation 370-410 nm; Emission 429-462 nm Green: Excitation 473-491 nm; Emission 502-561 nm Red: Excitation 580-598 nm; Emission 612-680 nm |
| Camera | High Sensitivity Monochrome CMOS Sensor; C-mount |
| Image Formats | JPG, BMP, TIF, GIF, or PNG |
| Image Size | 100 x 100 to 1900 x 1900 pixels |
| Field of View | 0.9 x 0.9 mm with 20x objective |
| Video Rates | Up to 10 frames/second (fps); with reduced frame size, up to 30 fps |
| Automated XY Stage | SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma) |
| Subdeck | No automation; most flasks, dishes, other |
| Stage Travel Range | 110 mm x 74 mm |
| Stage Move Speed | 25 mm/sec |
| Well-to-well time (seconds) | Image 96 wells: a) 1 color focus & image, 13 min, b) 3 color focus & image, 34 min |
| Computer Requirements | Windows 7, 8, 8.1, 10; 2 monitors recommended |
| Automation Friendly | SDK available |
| Power Requirements | USB for Lumascope; 100-240 V, 50-60 Hz for AutoStage |
| Dimensions, With Phase | 37.4 cm W x 43.8 cm D x 46.8 cm H [14.7 in W x 17.3 in D x 18.4 in H] |
| Weight | 13 kg (29 lb) without phase; 14 kg (31 lb) with phase |
| Operating Conditions | 4°C - 42°C, 5% - 95% RH non-condensing |