With Etaluma’s high resolution, versatile, and compact inverted LS Microscopes that actually fit inside your incubator, you get stable temperatures and CO2 levels throughout your assays.
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Or call 760-298-2355.
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Quality comparable to traditional, high-cost microscopes
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Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
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3-Color fluorescence, phase contrast, brightfield
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Images, time-lapse & live video
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1.25-100x objectives
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LS720 pictured above with phase contrast: Auto XY stage & autofocus
LS620
LS620: Blue, Green & Red Fluorescence with Manual Stage
LS Microscopes (previously called Lumascopes) utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by its USB connection, the LS620 easily records your photos, time-lapse series, and videos directly to your computer. And its compact size enables working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations.
LS720: Blue, Green, & Red Fluorescence and Walk-Away Automation
LS720
The powerful, new LS720 adds walk-away automation to the many features and high performance of the flagship 3-color LS620. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your microscopy experiments and high content screens. Place the LS720 in your incubator and you have a live cell imaging system at a fraction of the cost of conventional HCS systems. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a 48 hour time-lapse, the 720 offers a whole new world of automated microscopy!
Features and Benefits
- Automated XY stage with autofocus in Z (LS720) or manual stage (LS620) provide images (photos), time-lapse series, and videos recorded directly to your computer
- Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
- Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
- Robust software allows set-up and control across many locations, including microplates and custom arrays
- Versatile and compact design enables use inside cell culture incubators and hoods
- Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
- Flip-up deck (LS720) or Accessible deck (LS620) allows easy objective access
- Used manually, but also robot compatible (LS720; RS485, RS232, 5V digital interfaces)
- Objective compatibility with standard lenses permits use of your own objectives
Both utilize a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter.
Excitation and Emission Profiles of LS720 and LS620 filters
| Comparison of Lumascope Models | |||
|---|---|---|---|
| Features | LS560 | LS620 | LS720 |
| Optics | Green fluorescence; brightfield | Blue, green & red fluorescence; brightfield | Blue, green & red fluorescence; brightfield |
| Phase Contrast | Phase contrast optional (Olympus) | Phase contrast optional (Olympus) | Phase contrast optional (Olympus) |
| Objective Options | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
| Objective Compatibilities | RMS-threaded, infinity corrected, 45 mm parfocal distance | RMS-threaded, infinity corrected, 45 mm parfocal distance | RMS-threaded, infinity corrected, 45 mm parfocal distance |
| Fluorescence Filters | Green: Ex 457-493, Em 508-552 nm | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
| Camera | High Sensitivity Monochrome CMOS Sensor; C-mount | High Sensitivity Monochrome CMOS Sensor; C-mount | High Sensitivity Monochrome CMOS Sensor; C-mount |
| Image Formats | JPG, BMP, TIF, or PNG | JPG, BMP, TIF, or PNG | JPG, BMP, TIF, or PNG |
| Image Size | 100x100 to 1700x1700 pixels | 100x100 to 1900x1900 pixels | 100x100 to 1900x1900 pixels |
| Field of View | Up to 0.78 x 0.78 mm at 20x | Up to 0.78 x 0.78 mm at 20x | Up to 0.78 x 0.78 mm at 20x |
| Video Rates | Up to 10 fps; with reduced frame size, up to 30 fps | Up to 10 fps; with reduced frame size, up to 30 fps | Up to 10 fps; with reduced frame size, up to 30 fps |
| Stage | Manual XY | Manual XY | Automated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma) |
| Subdeck | NA | NA | No automation; most flasks, dishes, other |
| Well-to-well time (seconds) | NA | NA | Image 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min |
| Computer Requirements | Windows 7, 8, 8.1, 10; Core i5, SSD | Windows 7, 8, 8.1, 10; Core i5, SSD | Windows 7, 8, 8.1, 10; Core i5, SSD |
| Automation Friendly | SDK available | SDK available | SDK available |
| Power Requirements | USB | USB | USB for LS; 100-240 V, 50-60 Hz for autostage |
| Dimensions (WxDxH) | 24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches) | 24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches) | 37.4 x 43.8 x 23.9 cm (14.7 x 17.3 x 9.4 inches) |
| Weight | 3.25 kg (7.2 lb) | 3.25 kg (7.2 lb) | 11 kg (25 lb) |
| Operating Conditions | 0°C - 42°C, 5% - 95% RH non-condensing | 0°C - 42°C, 5% - 95% RH non-condensing | 0°C - 42°C, 5% - 95% RH non-condensing |