In Your Incubator
Cardiomyocyte Live Cell Microscopy!
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Live video of beating iPSC human cardiac myocytes
Beating pluripotent stem cell-derived cardiomyocytes
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LS850 Microscope
Blue, Green, & Red Fluorescence and Walk-Away Automation

Powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and offers the latest advances in optics, cameras, throughput, and user flexibility. . The LS850 improves the image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your automated microscopy experiments.  The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!

LS720
LS820 Microscope
Blue, Green & Red Fluorescence with Manual Stage

The powerful, new LS820 Microscope is the latest generation of our fully automated three-channel motorized Z model and offers the latest advances in optics, camera, and user flexibility. The LS820 provides the image quality, Z-range, illumination, and software flexibility over the manual LS620 model. Motorized focus allows autofocus and z-stacks over 14 mm with 100 nm steps. Easy-to-configure software generating user readable and editable scripts, facilitate your automated microscopy experiments. The LS820 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging flasks, dishes or slides with 3 fluorophores in a multi-day time-lapse, the LS820 offers a compact solution for walk-away automated microscopy!

LS620
Features and Benefits
  • Automated XY stage with autofocus in Z provides images (photos), time-lapse series, and videos recorded directly to your computer
  • Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
  • Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
  • Robust software allows set-up and control across many locations, including microplates and custom arrays
  • Versatile and compact design enables use inside cell culture incubators and hoods
  • Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
  • Flip-up deck allows easy objective access
  • Used manually, but also robot compatible (RS485, RS232, 5V digital interfaces)
  • Objective compatibility with standard lenses permits use of your own objectives
Both utilize a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The 720 also contains the same CMOS sensor as the LS620 that is larger than the sensor used in LS500 and LS400.
Excitation and Emission Profiles of LS720 and LS620 filters
Semrock 620 chart

Comparison of Lumascope Models
FeaturesLS560LS620LS720
OpticsGreen fluorescence; brightfieldBlue, green & red fluorescence; brightfieldBlue, green & red fluorescence; brightfield
Phase ContrastPhase contrast optional (Olympus)Phase contrast optional (Olympus)Phase contrast optional (Olympus)
Objective Options1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersGreen: Ex 457-493, Em 508-552 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm
CameraHigh Sensitivity Monochrome CMOS Sensor; C-mountHigh Sensitivity Monochrome CMOS Sensor; C-mountHigh Sensitivity Monochrome CMOS Sensor; C-mount
Image FormatsJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNG
Image Size100x100 to 1700x1700 pixels100x100 to 1900x1900 pixels100x100 to 1900x1900 pixels
Field of ViewUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20x
Video RatesUp to 10 fps; with reduced frame size, up to 30 fpsUp to 10 fps; with reduced frame size, up to 30 fpsUp to 10 fps; with reduced frame size, up to 30 fps
StageManual XYManual XYAutomated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma)
SubdeckNANANo automation; most flasks, dishes, other
Well-to-well time (seconds)NANAImage 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min
Computer RequirementsWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSD
Automation FriendlySDK availableSDK availableSDK available
Power RequirementsUSBUSBUSB for LS; 100-240 V, 50-60 Hz for autostage
Dimensions (WxDxH)24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches)24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches)37.4 x 43.8 x 23.9 cm (14.7 x 17.3 x 9.4 inches)
Weight3.25 kg (7.2 lb)3.25 kg (7.2 lb)11 kg (25 lb)
Operating Conditions0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing

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