High-Res Fluorescence Microscopes – Live Cell Imaging In Your Incubator

With Etaluma’s high resolution, versatile, and compact inverted LS Microscopes that actually fit inside your incubator, you get stable temperatures and CO2 levels throughout your assays.

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Or call 760-298-2355.
  • Quality comparable to traditional, high-cost microscopes
  • Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
  • 3-Color fluorescence, phase contrast, brightfield
  • Images, time-lapse & live video
  • 1.25-100x objectives
  • LS720 pictured above with phase contrast: Auto XY stage & autofocus

    LS620

      
LS620:  Blue, Green & Red Fluorescence with Manual Stage

LS Microscopes (previously called Lumascopes) utilize LED light sources, Semrock filters, advanced optical engineering, and CMOS sensors to provide near diffraction-limited (theoretical maximum) resolution. Powered only by its USB connection, the LS620 easily records your photos, time-lapse series, and videos directly to your computer. And its compact size enables working in challenging locations, including inside incubators, biological safety cabinets, and environmental control workstations.

 
LS720:  Blue, Green, & Red Fluorescence and Walk-Away Automation

LS720

The powerful, new LS720 adds walk-away automation to the many features and high performance of the flagship 3-color LS620. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your microscopy experiments and high content screens. Place the LS720 in your incubator and you have a live cell imaging system at a fraction of the cost of conventional HCS systems. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a 48 hour time-lapse, the 720 offers a whole new world of automated microscopy!

 
 
 
 
 
 
Features and Benefits
  • Automated XY stage with autofocus in Z (LS720) or manual stage (LS620) provide images (photos), time-lapse series, and videos recorded directly to your computer
  • Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
  • Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
  • Robust software allows set-up and control across many locations, including microplates and custom arrays
  • Versatile and compact design enables use inside cell culture incubators and hoods
  • Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
  • Flip-up deck (LS720) or Accessible deck (LS620) allows easy objective access
  • Used manually, but also robot compatible (LS720; RS485, RS232, 5V digital interfaces)
  • Objective compatibility with standard lenses permits use of your own objectives

Both utilize a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The 720 also contains the same CMOS sensor as the LS620 that is larger than the sensor used in LS500 and LS400.

 
Excitation and Emission Profiles of LS720 and LS620 filters

Semrock 620 chart

 

 

 

 

 

 

 

 

Comparison of Lumascope Models
FeaturesLS560LS620LS720
OpticsGreen fluorescence; brightfieldBlue, green & red fluorescence; brightfieldBlue, green & red fluorescence; brightfield
Phase ContrastPhase contrast optional (Olympus)Phase contrast optional (Olympus)Phase contrast optional (Olympus)
Objective Options1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification
Objective CompatibilitiesRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distanceRMS-threaded, infinity corrected, 45 mm parfocal distance
Fluorescence FiltersGreen: Ex 457-493, Em 508-552 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nmBlue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm
CameraHigh Sensitivity Monochrome CMOS Sensor; C-mountHigh Sensitivity Monochrome CMOS Sensor; C-mountHigh Sensitivity Monochrome CMOS Sensor; C-mount
Image FormatsJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNGJPG, BMP, TIF, or PNG
Image Size100x100 to 1700x1700 pixels100x100 to 1900x1900 pixels100x100 to 1900x1900 pixels
Field of ViewUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20xUp to 0.78 x 0.78 mm at 20x
Video RatesUp to 10 fps; with reduced frame size, up to 30 fpsUp to 10 fps; with reduced frame size, up to 30 fpsUp to 10 fps; with reduced frame size, up to 30 fps
StageManual XYManual XYAutomated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma)
SubdeckNANANo automation; most flasks, dishes, other
Well-to-well time (seconds)NANAImage 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min
Computer RequirementsWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSDWindows 7, 8, 8.1, 10; Core i5, SSD
Automation FriendlySDK availableSDK availableSDK available
Power RequirementsUSBUSBUSB for LS; 100-240 V, 50-60 Hz for autostage
Dimensions (WxDxH)24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches)24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches)37.4 x 43.8 x 23.9 cm (14.7 x 17.3 x 9.4 inches)
Weight3.25 kg (7.2 lb)3.25 kg (7.2 lb)11 kg (25 lb)
Operating Conditions0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing0°C - 42°C, 5% - 95% RH non-condensing