Our Automated Incubator Microscopes
Live Cell Microscopy of Organoids
In Your Incubator
3D Live Cell Microscopy of Organoids in Your Incubator
With Etaluma’s high resolution, versatile, and compact inverted automated phase contrast and fluorescence microscopes that actually fit inside your incubator, you get stable temperatures and CO2 levels throughout your long term organoid assays.
- Quality comparable to traditional, high-cost microscopes
- Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
- 3-Color fluorescence, phase contrast, brightfield
- Images, time-lapse & live video
- 1.25-100x objectives
- LS850 pictured above with phase contrast: Auto XY stage & autofocus
- Z-stacking and deconvolution
- Large FOV with tiling
- Custom ROIs
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LS820 Microscope
Blue, Green & Red Fluorescence with Manual Stage
The powerful, new LS820 Microscope is the latest generation of our fully automated three-channel motorized Z model and offers the latest advances in optics, camera, and user flexibility. The LS820 provides the image quality, Z-range, illumination, and software flexibility over the manual LS620 model. Motorized focus allows autofocus and z-stacks over 14 mm with 100 nm steps. Easy-to-configure software generating user readable and editable scripts, facilitate your automated microscopy experiments. The LS820 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging flasks, dishes or slides with 3 fluorophores in a multi-day time-lapse, the LS820 offers a compact solution for walk-away automated microscopy!
LS850 Microscope
Blue, Green, & Red Fluorescence and Walk-Away Automation
Powerful, new LS850 Microscope is the latest generation of our fully automated three-channel flagship model and offers the latest advances in optics, cameras, throughput, and user flexibility. . The LS850 improves the image quality, motion speed, illumination, and software flexibility over the previous LS720 model. Exquisite XY motion control, motorized focus that allows autofocus and z-stacks, and easy-to-configure software combine to facilitate your automated microscopy experiments. The LS850 in your incubator allows you to have a live cell imaging system that offers minimum photo toxicity and the most stable environment for long term imaging. Whether imaging multiple fields in your flasks or 1536 wells of cells with 3 fluorophores in a multi-day time-lapse, the LS850 offers a whole new world of walk-away automated microscopy!
Features and Benefits
- Images, tiled images, Z-stacks, time-lapse series, and videos recorded directly to your computer
- Bright field and phase contrast transmitted illumination
- LED excitation and high performance optics provide near diffraction-limited (theoretical maximum) resolution
- Multi-OS software allows set-up and control across any locations, including microplates, microfluidic chips, slides, dishes, flasks, and deck-top chambers and custom arrays
- High speed stage with single digit micron precision
- Long range motorized Z for autofocus and Z-stacks down to 100 nm
- Compact and robust design enables use inside cell culture hoods, incubators, hypoxic chambers, and gloveboxes
- Detects blue, green and red fluorophores, including common probes such as Hoechst, DAPI, FITC, Fluo-4, GFP, Texas Red & mCherry GFP
- Open source Python control code available
- Objective compatibility with standard lenses and available with optional automated 4-objective turret
Excitation and Emission Profiles of LS850 and LS820 filters
| Comparison of Lumascope Models | ||
|---|---|---|
| Features | LS820 | LS850 |
| Optics | Blue, green & red fluorescence; bright field | Blue, green & red fluorescence; bright field |
| Phase Contrast | Phase contrast optional (Auraphase) | Phase contrast optional (Auraphase) |
| Objective Options | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
| Objective Compatibilities | RMS-threaded, infinity corrected, 45 mm parfocal distance | RMS-threaded, infinity corrected, 45 mm parfocal distance |
| Fluorescence Filters | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
| Camera | High QE Monochrome CMOS BSI Sensor; 4.4 Megapixel, 12-Bit | High QE Monochrome CMOS BSI Sensor; 4.4 Megapixel, 12-Bit |
| Image Formats | JPG, BMP, TIF, or PNG | JPG, BMP, TIF, or PNG |
| Image Size | 100x100 to 2100x2100 pixels | 100x100 to 2100x2100 pixels |
| Field of View | Up to 0.78 x 0.78 mm at 20x | Up to 0.78 x 0.78 mm at 20x |
| Video Rates | 45 fps (exposure limited) | 45 fps (exposure limited) |
| Stage | Manual XY | Automated XY, 110 mm X 74mm, 1-3 micron positional reproducibility |
| Motorized Z | 14mm travel, 100nm step, image-based autofocus, Z-stacks | 14mm travel, 100nm step, image-based autofocus, Z-stacks |
| Well-to-well time (seconds) | NA | Image 96 wells: under 2 minutes with 1 channel, no auto-focus |
| Control Software | LumaviewPro | LumaviewPro |
| Computer Requirements | Windows 10, 11; Core i7, SSD | Windows 10, 11; Core i7, 1 TB SSD, 8 GM RAM |
| Integration | Python Source under the MIT Open Source License | Python Source under the MIT Open Source License |
| Power Requirements | USB3 for LS; 100-240 V, 50-60 Hz for autostage | USB3 for LS; 100-240 V, 50-60 Hz for autostage |
| Dimensions (WxDxH) | 24cm W x 22.6cm D x 27.8cm H (9.4in W x 8.9in D x 10.9in H) | 39.8cm W x 29.7cm D x 29.7cm H (15.6in W x 11.6in D x 11.6in H) |
| Weight | 5 kg (10 lb) | 9.5 kg (21 lb) |
| Operating Conditions | 0°C - 42°C, 5% - 95% RH non-condensing | 0°C - 42°C, 5% - 95% RH non-condensing |
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