Lumascopes

Applications: From Time-Lapse Microscopy to High Content Screening

Compact and versatile, the LS850 brings high content screening to the benchtop while still excelling at live-cell imaging in the incubator.

A Short Demo of the LS850 Microscope from Etaluma

Etaluma has long been associated with time-lapse microscopy inside incubators — providing stable, robust conditions for live-cell imaging. That’s still a cornerstone of how many labs use our microscopes.

But our community of users is doing much more. Increasingly, we’re seeing our Lumascope systems used on the benchtop for fixed cells and immunofluorescence assays. These applications open up new ways to leverage Etaluma platforms outside the incubator, where ease-of-use and flexibility are critical.

In this short demo, Etaluma co-founder Brian Rasnow shows what benchtop High Content Screening looks like with the LS850. The system’s compact footprint and straightforward workflow make it possible to capture high-quality, multi-channel images without the need for complex infrastructure.

From incubator-based time-lapse to benchtop immunofluorescence, the LS850 illustrates how accessible high content imaging can be.

See Other Use Cases and Features of our Lumascopes

Live cell imaging

See Etaluma – Cardiac Myocytes Undergoing Division

Cell growth and confluence

See Time Lapse Video of MSC in 2D Cell Culture

Cell migration and wound healing

See Cell Migration & Wound Healing Application Note
See Migration of MSC in 2D Cell Culture

Cell cycle protein expression

See Human HT1080 Fibrosarcoma Cells with LS600

Use of micro-environmental systems

See Bioptechs products on Etaluma LS500

Calcium assays

GCAMP5 activity in a sensory neuron

Determining transfection efficiency

See HUVEC Transfection Optimized with Cytofect™ Kit

In Vitro Exercise Model

Cultured skeletal muscle myotubes are electrically stimulated under hypoxic conditions and with temperature manipulations. Cell signal transduction dynamics are measured using proteomic techniques to help understand how exercise stressors are translated into fitness-promoting adaptions such as increased mitochondria. Probe in photo measures PO2 in the cell medium rather than in the atmosphere. LS620 allows visualization of contracting cells and assessment of their health.

Thank you to Dr David Clarke and his lab, Laboratory for Quantitive Exercise Biology, Simon Fraser University, British Columbia, Canada

Behavior of stem cells

See Etaluma-Human Neural Stem Cells in Culture 1
See Etaluma-Human Neural Stem Cells in Culture 2
Also see reattachment of neuronal stem cells passaged with Accutase (scroll down to see video)

Cell death assays

See Lumascope – BF Cell Death

Apoptosis induction

Spheroid development and behavior

See 3D Spheroid Formation of MSC
See Spheroid-Migration of MSC in a PEG-Fibrinogen Hydrogel

Cultivation of yeast

See Cultivation of S. cerevisiae in Core-Shell Microcapsules

Intravital studies

See Series: Neutrophil migration intravital mouse imaging

Study of lower eukaryotes

See Mosquito larva on Etaluma

Photomicroscopy in locations without AC power

See Lumascope with 20x at Boiling Springs Lake

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