Lumaquant Image Analysis Software
- Powerful solution for analyzing cell-based assays
- Analyze time-lapse images, e.g., proliferation & migration
- Based on DRVisions’ Aivia program
- For more info, see Lumaquant.
Microvolution Deconvolution Software
- Microvolution’s “real-time” deconvolution
- Provides confocal-like images using wide-field optics
- The new “Software Confocal”!
- For more info, see Microvolution Deconvolution.
Request Info
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Or Call 760-298-2355.
Live Cell Imaging In Your Incubator!
- Quality comparable to traditional, high-cost microscopes
- Compatible with microplates, dishes, chamber slides, flasks, microfluidic chips
- 3-Color fluorescence, phase contrast, brightfield
- Images, time-lapse & live video
- 1.25-100x objectives
- LS720 pictured above with phase contrast: Auto XY stage & autofocus
LS720 Automated Fluorescence Microscope
Features and Benefits
- Automated XY stage with autofocus in Z (LS720) or manual stage (LS620) provide images (photos), time-lapse series, and videos recorded directly to your computer
- Fully functioning microscope empowers users to visualize cells from microplates, flasks, slides or custom labware
- Modern LED and advanced optical design provide near diffraction-limited (theoretical maximum) resolution
- Robust software allows set-up and control across many locations, including microplates and custom arrays
- Versatile and compact design enables use inside cell culture incubators and hoods
- Detects blue, green and red fluorophores, including BFP, DAPI, FITC, Fluo-4, GFP & mCherry
- Flip-up deck (LS720) or Accessible deck (LS620) allows easy objective access
- Used manually, but also robot compatible (LS720; RS485, RS232, 5V digital interfaces)
- Objective compatibility with standard lenses permits use of your own objectives
Both utilize a Semrock Brightline® Pinkel filter set optimized for 405 nm, 488 nm, and 594 nm LED light sources. The 5-filter set is comprised of 3 exciting filters, 1 triple-band emitting filter, and 1 beam-splitting filter. The 720 also contains the same CMOS sensor as the LS620 that is larger than the sensor used in LS500 and LS400.
Excitation and Emission Profiles of LS720 and LS620 filters
Comparison of Lumascope Models | |||
---|---|---|---|
Features | LS560 | LS620 | LS720 |
Optics | Green fluorescence; brightfield | Blue, green & red fluorescence; brightfield | Blue, green & red fluorescence; brightfield |
Phase Contrast | Phase contrast optional (Olympus) | Phase contrast optional (Olympus) | Phase contrast optional (Olympus) |
Objective Options | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification | 1.25x,2.5x, 4x, 10x, 20x, 40x, 60x, and 100x(oil) magnification |
Objective Compatibilities | RMS-threaded, infinity corrected, 45 mm parfocal distance | RMS-threaded, infinity corrected, 45 mm parfocal distance | RMS-threaded, infinity corrected, 45 mm parfocal distance |
Fluorescence Filters | Green: Ex 457-493, Em 508-552 nm | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm | Blue: Ex 370-410, Em 429-462 nm; Green: Ex 473-491, Em 502-561 nm; Red: Ex 580-598, Em 612-680 nm |
Camera | High Sensitivity Monochrome CMOS Sensor; C-mount | High Sensitivity Monochrome CMOS Sensor; C-mount | High Sensitivity Monochrome CMOS Sensor; C-mount |
Image Formats | JPG, BMP, TIF, or PNG | JPG, BMP, TIF, or PNG | JPG, BMP, TIF, or PNG |
Image Size | 100x100 to 1700x1700 pixels | 100x100 to 1900x1900 pixels | 100x100 to 1900x1900 pixels |
Field of View | Up to 0.78 x 0.78 mm at 20x | Up to 0.78 x 0.78 mm at 20x | Up to 0.78 x 0.78 mm at 20x |
Video Rates | Up to 10 fps; with reduced frame size, up to 30 fps | Up to 10 fps; with reduced frame size, up to 30 fps | Up to 10 fps; with reduced frame size, up to 30 fps |
Stage | Manual XY | Manual XY | Automated XYZ, SBS nest, 6- to 1536-well microplates; microfluidic chambers (contact Etaluma) |
Subdeck | NA | NA | No automation; most flasks, dishes, other |
Well-to-well time (seconds) | NA | NA | Image 96 wells: a) 1 color focus & image: 13 min; b) 3 color focus & image: 34 min |
Computer Requirements | Windows 7, 8, 8.1, 10; Core i5, SSD | Windows 7, 8, 8.1, 10; Core i5, SSD | Windows 7, 8, 8.1, 10; Core i5, SSD |
Automation Friendly | SDK available | SDK available | SDK available |
Power Requirements | USB | USB | USB for LS; 100-240 V, 50-60 Hz for autostage |
Dimensions (WxDxH) | 24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches) | 24 x 14 x 16.5 cm (9.4 x 5.5 x 6.5 inches) | 37.4 x 43.8 x 23.9 cm (14.7 x 17.3 x 9.4 inches) |
Weight | 3.25 kg (7.2 lb) | 3.25 kg (7.2 lb) | 11 kg (25 lb) |
Operating Conditions | 0°C - 42°C, 5% - 95% RH non-condensing | 0°C - 42°C, 5% - 95% RH non-condensing | 0°C - 42°C, 5% - 95% RH non-condensing |